Device

Part:BBa_K4461512:Design

Designed by: CHANG, YU-TANG   Group: iGEM22_NYCU-Taipei   (2022-10-10)


BBa_K4461501, BBa_K4461504, BBa_K4461508


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use enzyme digestion to get backbone pSB1C3 with BBa_K4461504, also we use enzyme digestion to get two devices BBa_K4461501 and BBa_K4461508. Then, we ligate three fragments in one time.


Source

gene synthesis

References